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J Pharm Biomed Anal. 2016 Jun 5;125:400-7. doi: 10.1016/j.jpba.2016.04.029. Epub 2016 Apr 20.

Qualitative and quantitative analysis of chemical constituents of Centipeda minima by HPLC-QTOF-MS & HPLC-DAD.

Author information

1
State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
2
State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China.
3
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
4
State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address: sibao.chen@polyu.edu.hk.
5
State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address: daniel.mok@polyu.edu.hk.

Abstract

A high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) method in both positive and negative ion modes was established to investigate the major constituents in the ethanolic extract of Centipeda minima (EBSC). Twelve common components including flavones and their glycosides, phenolic and polyphenolic acids, and sesquiterpene lactone were identified in ten batches of samples based on comparison with the retention time and accurate mass of external standards (mass accuracy within 3ppm) or the fragmentation patterns of tandem MS. Meanwhile, a simple, accurate and reliable HPLC-DAD method was also developed to determine the content of 10 chemical markers simultaneously. Results obtained from method validations including linearity, accuracy and precision showed that this new method is reliable and robust. Isochlorogenic acid A and brevilin A were found to be the most abundant in the ethanol extract of EBSC and could be served as markers for quality control of EBSC.

KEYWORDS:

Brevilin A; Centipeda minima; Herbal analysis; Isochlorogenic acid A; Quadrupole time-of-flight mass spectrometry (QTOF MS); Quality control

PMID:
27131150
DOI:
10.1016/j.jpba.2016.04.029
[Indexed for MEDLINE]

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