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Aquat Toxicol. 2016 Jul;176:97-105. doi: 10.1016/j.aquatox.2016.04.019. Epub 2016 Apr 22.

The different response mechanisms of Wolffia globosa: Light-induced silver nanoparticle toxicity.

Author information

1
Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China.
2
Key Laboratory of Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China.
3
Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China; Ningbo Research Center for Urban Environment, Chinese Academy of Sciences, Ningbo, China. Electronic address: hwzhang@iue.ac.cn.

Abstract

Silver nanoparticles (AgNPs) have emerged as a promising bactericide. Plants are a major point of entry of contaminants into trophic chains. Here, the physiological responses of Wolffia globosa to AgNPs have been probed using different light schemes, and these data may reveal new insights into the toxic mechanism of AgNPs. W. globosa was grown in culture medium and treated with different concentrations of AgNPs for 24h under pre- and post-illuminated conditions. However, fluorescence quenching, the accumulation of sugar and the reduction of Hill reaction activity were found in response to the AgNP-stresses. In the pre-illuminated condition, oxidative damage was obvious, as indicated by the higher malondialdehyde (MDA) content and an up-regulation of superoxide dismutase (SOD) activity. The maximum increases of MDA content and SOD activity were 1.14 and 2.52 times the respective controls when exposed to 10mg/L AgNPs. In contrast, in the post-illuminated condition, the alterations in photosynthetic pigment and soluble proteins content were more significant than the alterations in oxidative stress. The contents of chlorophyll a, carotenoids and soluble protein decreased to 77.7%, 66.2% and 72.9% of the controls after treatment with the highest concentration of AgNPs (10mg/L). Based on the different physiological responses, we speculated that in the pre-illuminated condition, oxidative stress was responsible for the decline in the oxygen evolution rate, while in the post-illuminated condition, the decrease in the Hill reaction activity could be attributed to the blocking of electron transfer and an insufficient proton supply. Our findings demonstrate that environmental factors regulate the physiological responses of plants to AgNPs through distinct mechanisms.

KEYWORDS:

Illumination; Physiological responses; Silver nanoparticles; Wolffia globosa

PMID:
27130969
DOI:
10.1016/j.aquatox.2016.04.019
[Indexed for MEDLINE]

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