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Virus Res. 2016 Jul 15;220:129-35. doi: 10.1016/j.virusres.2016.04.014. Epub 2016 Apr 26.

Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses.

Author information

1
Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
2
Komipharm Co., Ltd., Republic of Korea.
3
Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea; Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea; Komipharm Co., Ltd., Republic of Korea.
4
Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea; Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea. Electronic address: shin0089@cnu.ac.kr.

Abstract

In this study, we evaluated the immune responses of avian metapneumovirus harboring chicken Fc molecule. Stable Vero cells expressing chicken Fc chimera on its surface (Vero-cFc) were established, and we confirmed that aMPV grown in Vero-cFc incorporated host derived chimera Fc into the aMPV virions. Immunization of chicken with aMPV-cFc induced higher level of antibodies and inflammatory cytokines; (Interferon (IFN)-γ and Interleukin (IL)-1β) compared to those of aMPV. The increased levels of antibodies and inflammatory cytokines in chicken immunized with aMPV-cFc were statistically significantly (p<0.05) to that of aMPV and control. The aMPV-cFc group also generated the highest neutralizing antibody response. After challenges, chickens immunized with aMPV-cFc showed much less pathological signs in nasal turbinates and trachea so that we could confirm aMPV-cFc induced higher protection than that of aMPV. The greater ability of aMPV harboring chicken Fc to that of aMPV presented it as a possible vaccine candidate.

KEYWORDS:

Antibody response; Avian metapneumovirus (aMPV); Chicken Fc; Cytokines; Neutralizing antibodies; Vaccines

PMID:
27130629
DOI:
10.1016/j.virusres.2016.04.014
[Indexed for MEDLINE]

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