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Ann Neurol. 2016 Jul;80(1):160-5. doi: 10.1002/ana.24679. Epub 2016 Jun 1.

Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt-jakob disease: An international study.

Author information

1
National CJD Research & Surveillance Unit, Western General Hospital, University of Edinburgh, Edinburgh, Scotland, United Kingdom.
2
Department of Neurological Sciences, National Institute of Health, Rome, Italy.
3
Institute of Neurological Sciences, Scientific Institute for Research, Hospitalization and Health Care, Bologna, Italy.
4
Department of Neurodegenerative Diseases, Scientific Institute for Research, Hospitalization and Health Care, Carlo Besta Neurological Institute, Milan, Italy.
5
Sorbonne Universities, Pierre and Marie Curie University, Brain and Spine Institute, Paris, France.
6
National Reference Centre for Unconventional Transmissible Agents, Paris, France.
7
Center for Neuropathology and Prion Research, Ludwig Maximilian University, Munich, Germany.
8
Department of Neurology, Institute of Born Bunge, University of Antwerp, Antwerp, Belgium.
9
Florey Institute of Neuroscience and Mental Health, University of Melbourne, Melbourne, Victoria, Australia.
10
Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
11
Prion Laboratory Section, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
12
Department of Neurology, University Medical Center and German Center for Neurodegenerative Diseases, University of Göttingen, Göttingen, Germany.
13
Department of Medicine, University of Melbourne, Melbourne, Victoria, Australia.
14
APHP, Pitié-Salpêtrière Hospital, Paris, France.
15
Department of Biomedical and Neuromotor Sciences, University of Bologna, Italy.

Abstract

Real-time quaking-induced conversion (RT-QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt-Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT-QuIC is a suitably reliable and robust technique for clinical practice. Ann Neurol 2016;80:160-165.

PMID:
27130376
PMCID:
PMC4982084
DOI:
10.1002/ana.24679
[Indexed for MEDLINE]
Free PMC Article

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