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Ann Biomed Eng. 2016 Oct;44(10):2913-2921. doi: 10.1007/s10439-016-1629-z. Epub 2016 Apr 29.

Cationic Contrast Agent Diffusion Differs Between Cartilage and Meniscus.

Author information

1
Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland. juuso.honkanen@uef.fi.
2
Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland. juuso.honkanen@uef.fi.
3
Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland.
4
Department of Pharmacology, Boston University School of Medicine, Boston, MA, USA.
5
Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland.
6
Department of Diagnostic Radiology, Oulu University Hospital, Oulu, Finland.
7
Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland.
8
Department of Biomedical Engineering, Boston University, Boston, MA, USA.
9
Department of Chemistry, Boston University, Boston, MA, USA.
10
Department of Integrative Medical Biology, University of Umea, Umeå, Sweden.
11
Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland.

Abstract

Contrast enhanced computed tomography (CECT) is a non-destructive imaging technique used for the assessment of composition and structure of articular cartilage and meniscus. Due to structural and compositional differences between these tissues, diffusion and distribution of contrast agents may differ in cartilage and meniscus. The aim of this study is to determine the diffusion kinematics of a novel iodine based cationic contrast agent (CA(2+)) in cartilage and meniscus. Cylindrical cartilage and meniscus samples (d = 6 mm, h ≈ 2 mm) were harvested from healthy bovine knee joints (n = 10), immersed in isotonic cationic contrast agent (20 mgI/mL), and imaged using a micro-CT scanner at 26 time points up to 48 h. Subsequently, normalized X-ray attenuation and contrast agent diffusion flux, as well as water, collagen and proteoglycan (PG) contents in the tissues were determined. The contrast agent distributions within cartilage and meniscus were different. In addition, the normalized attenuation and diffusion flux were higher (p < 0.05) in cartilage. Based on these results, diffusion kinematics vary between cartilage and meniscus. These tissue specific variations can affect the interpretation of CECT images and should be considered when cartilage and meniscus are assessed simultaneously.

KEYWORDS:

Computed tomography; Contrast enhancement; Diffusion flux; Diffusion kinematics; Normalized attenuation

PMID:
27129372
PMCID:
PMC5042996
DOI:
10.1007/s10439-016-1629-z
[Indexed for MEDLINE]
Free PMC Article

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