Format

Send to

Choose Destination
J Cell Sci. 2016 Jun 15;129(12):2343-53. doi: 10.1242/jcs.185546. Epub 2016 Apr 28.

The EZH1-SUZ12 complex positively regulates the transcription of NF-κB target genes through interaction with UXT.

Author information

1
Department of Biochemistry and Molecular Biology, College of Life Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University, Wuhan, Hubei 430072, China.
2
Department of Biochemistry and Molecular Biology, College of Life Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University, Wuhan, Hubei 430072, China wumin@whu.edu.cn lilianyun@whu.edu.cn.

Abstract

Unlike other members of the polycomb group protein family, EZH1 has been shown to positively associate with active transcription on a genome-wide scale. However, the underlying mechanism for this behavior still remains elusive. Here, we report that EZH1 physically interacts with UXT, a small chaperon-like transcription co-activator. UXT specifically interacts with EZH1 and SUZ12, but not EED. Similar to upon knockdown of UXT, knockdown of EZH1 or SUZ12 through RNA interference in the cell impairs the transcriptional activation of nuclear factor (NF)-κB target genes induced by TNFα. EZH1 deficiency also increases TNFα-induced cell death. Interestingly, chromatin immunoprecipitation and the following next-generation sequencing analysis show that H3K27 mono-, di- and tri-methylation on NF-κB target genes are not affected in EZH1- or UXT-deficient cells. EZH1 also does not affect the translocation of the p65 subunit of NF-κB (also known as RELA) from the cytosol to the nucleus. Instead, EZH1 and SUZ12 regulate the recruitment of p65 and RNA Pol II to target genes. Taken together, our study shows that EZH1 and SUZ12 act as positive regulators for NF-κB signaling and demonstrates that EZH1, SUZ12 and UXT work synergistically to regulate pathway activation in the nucleus.

KEYWORDS:

EZH1; Histone methylation; NF-κB signaling pathway; Transcription regulation; UXT

PMID:
27127229
DOI:
10.1242/jcs.185546
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center