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Dent Traumatol. 2016 Oct;32(5):390-6. doi: 10.1111/edt.12272. Epub 2016 Apr 29.

The effect of Aloe vera gel on viability of dental pulp stem cells.

Author information

1
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran. mehrabad@sums.ac.ir.
3
Department of Regenerative Medicine, University of Manitoba, Winnipeg, MB, Canada. mehrabad@sums.ac.ir.
4
Department of Biology, Science and Research Branch, Islamic Azad University, Fars, Iran.

Abstract

BACKGROUND:

Dental pulp stem cells (DPSCs) can play a prominent role in tissue regeneration. Aloe vera L. (Liliaceae) contains the polysaccharide of acemannan that was shown to be a trigger factor for cell proliferation, differentiation, mineralization, and dentin formation.

AIM:

This study sought to determine the viability of DPSCs in Aloe vera in comparison with Hank's balanced salt solution (HBSS).

MATERIALS AND METHOD:

Twelve rabbits underwent anesthesia, and their incisor teeth were extracted; the pulp tissue was removed, chopped, treated with collagenase and plated in culture flasks. DPSCs from passage 3 were cultured in 24-well plates, and after 3 days, the culture media changed to 10, 25, 50, and 100% concentrations of Aloe vera at intervals of 45 and 90 min and 3 and 6 h. Distilled water was used as negative and HBSS as positive control for comparison. The cell morphology, viability, population doubling time (PDT), and growth kinetics were evaluated. RT-PCR was carried out for characterization and karyotyping for chromosomal stability.

RESULTS:

Aloe vera showed a significant higher viability than HBSS (74.74%). The 50% Aloe vera showed higher viability (97.73%) than other concentrations. PDT in 50% concentration was 35.1 h and for HBSS was 49.5 h. DPSCs were spindle shaped and were positive for CD73 and negative for CD34 and CD45. Karyotyping was normal.

CONCLUSIONS:

Aloe vera as an inexpensive and available herb can improve survival of avulsed or broken teeth in emergency cases as a transfer media.

KEYWORDS:

Aloe vera; Hank's balanced salt solution; dental pulp stem cells; viability

PMID:
27126516
DOI:
10.1111/edt.12272
[Indexed for MEDLINE]

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