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Spectrochim Acta A Mol Biomol Spectrosc. 2016 Aug 5;165:120-6. doi: 10.1016/j.saa.2016.04.040. Epub 2016 Apr 20.

Quantifying Aflatoxin B1 in peanut oil using fabricating fluorescence probes based on upconversion nanoparticles.

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School of Food and Biological engineering, Jiangsu University, Zhenjiang 212013, PR China.
Institute for Global Food Security, Queen's University Belfast, BT95GN, Northern Ireland, United Kingdom.
School of Food and Biological engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address:


Rare earth doped upconversion nanoparticles convert near-infrared excitation light into visible emission light. Compared to organic fluorophores and semiconducting nanoparticles, upconversion nanoparticles (UCNPs) offer high photochemical stability, sharp emission bandwidths, and large anti-Stokes shifts. Along with the significant light penetration depth and the absence of autofluorescence in biological samples under infrared excitation, these UCNPs have attracted more and more attention on toxin detection and biological labelling. Herein, the fluorescence probe based on UCNPs was developed for quantifying Aflatoxin B1 (AFB1) in peanut oil. Based on a specific immunity format, the detection limit for AFB1 under optimal conditions was obtained as low as 0.2ng·ml(-1), and in the effective detection range 0.2 to 100ng·ml(-1), good relationship between fluorescence intensity and AFB1 concentration was achieved under the linear ratios up to 0.90. Moreover, to check the feasibility of these probes on AFB1 measurements in peanut oil, recovery tests have been carried out. A good accuracy rating (93.8%) was obtained in this study. Results showed that the nanoparticles can be successfully applied for sensing AFB1 in peanut oil.


Biological labelling; Fluorescence probes; Rapid toxin detection; Upconversion nanoparticles

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