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PLoS Genet. 2016 Apr 28;12(4):e1005995. doi: 10.1371/journal.pgen.1005995. eCollection 2016 Apr.

shRNA-Based Screen Identifies Endocytic Recycling Pathway Components That Act as Genetic Modifiers of Alpha-Synuclein Aggregation, Secretion and Toxicity.

Author information

1
Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisboa, Portugal.
2
CEDOC, Chronic Diseases Research Center, NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Lisboa, Portugal.
3
Instituto de Tecnologia Química e Biológica, Universidade Nova de lisboa, Oeiras, Portugal.
4
Instituto Gulbenkian de Ciência, Oeiras, Portugal.
5
Department of Genetics, University of Leicester, Leicester, United Kingdom.
6
Department of Neurodegeneration and Restorative Research, University Medical Center Göttingen, Göttingen, Germany.
7
Max Planck Institute for Experimental Medicine, Göttingen, Germany.

Abstract

Alpha-Synuclein (aSyn) misfolding and aggregation is common in several neurodegenerative diseases, including Parkinson's disease and dementia with Lewy bodies, which are known as synucleinopathies. Accumulating evidence suggests that secretion and cell-to-cell trafficking of pathological forms of aSyn may explain the typical patterns of disease progression. However, the molecular mechanisms controlling aSyn aggregation and spreading of pathology are still elusive. In order to obtain unbiased information about the molecular regulators of aSyn oligomerization, we performed a microscopy-based large-scale RNAi screen in living cells. Interestingly, we identified nine Rab GTPase and kinase genes that modulated aSyn aggregation, toxicity and levels. From those, Rab8b, Rab11a, Rab13 and Slp5 were able to promote the clearance of aSyn inclusions and rescue aSyn induced toxicity. Furthermore, we found that endocytic recycling and secretion of aSyn was enhanced upon Rab11a and Rab13 expression in cells accumulating aSyn inclusions. Overall, our study resulted in the identification of new molecular players involved in the aggregation, toxicity, and secretion of aSyn, opening novel avenues for our understanding of the molecular basis of synucleinopathies.

PMID:
27123591
PMCID:
PMC4849646
DOI:
10.1371/journal.pgen.1005995
[Indexed for MEDLINE]
Free PMC Article

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