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Adv Pharm Bull. 2016 Mar;6(1):83-90. doi: 10.15171/apb.2016.013. Epub 2016 Mar 17.

Preparation and Characterization of Nanosuspension of Aprepitant by H96 Process.

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Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal.
Dr. Reddy's Laboratories Ltd., Hyderabad, India.



Nanosuspension in drug delivery is known to improve solubility, dissolution and eventually bioavailability of the drugs. The purpose of the study was to compare particle size of nanosuspensions prepared by the first generation approach and H96 approach and to evaluate the effectiveness of H96 approach.


The nanosuspension of aprepitant was prepared by HPH and H96 approach. The prepared nanosuspensions were characterized for their particle size and zeta potential. The optimized nanosuspension was further evaluated for DSC, FT-IR, solubility and dissolution.


The optimized nanosuspension (NCLH5) prepared using combination of tween 80 and poloxamer 188 as stabilizer, showed particle size of 35.82 nm and improved solubility and dissolution profile over pure drug. NCLH5 was chosen optimized formulation and further evaluated for other parameters after lyophilization. Lyophilization resulted in increase in particle size. The solubility and dissolution studies showed favorable increase in the performance. The FT-IR and DSC analysis showed change in the crystallinity after nanosizing.


The observations indicated that lyophilization prior to high pressure homogenization resulted in efficient particle size reduction yielding smaller particles than first generation preparation technique. H96 is a good and easy alternative to achieve efficient particle size reduction of drug in lesser time and increase its solubility and dissolution.


High pressure homogenization; Lyophilization; Nanosuspension; Particle size; Second generation approach

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