Format

Send to

Choose Destination
Genome Biol. 2016 Apr 28;17:77. doi: 10.1186/s13059-016-0938-8.

CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq.

Author information

1
Department of Biology, Technion - Israel Institute of Technology, Haifa, Israel.
2
Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
3
Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, 02142, USA.
4
Department of Biology, MIT, Cambridge, MA, 02139, USA.
5
Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02140, USA.
6
Fluidigm Corporation, 7000 Shoreline Court, Suite 100, South San Francisco, CA, 94080, USA.
7
Department of Biology, Technion - Israel Institute of Technology, Haifa, Israel. yanai@technion.ac.il.

Abstract

Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with threefold higher sensitivity, lower costs, and less hands-on time. We implemented CEL-Seq2 on Fluidigm's C1 system, providing its first single-cell, on-chip barcoding method, and we detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2's increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use.

PMID:
27121950
PMCID:
PMC4848782
DOI:
10.1186/s13059-016-0938-8
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center