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Biomarkers. 2016 Apr 28:1-9. [Epub ahead of print]

microRNA signatures in peripheral blood fail to detect acute cellular rejection after liver transplantation.

Author information

1
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany ;
2
b Department of Visceral, Transplantation, Thoracic, and Vascular Surgery , University of Leipzig , Leipzig , Germany ;
3
c Department of Neonatology , Charité - Universitätsmedizin Berlin , Germany ;
4
d Department of Translational Centre for Regenerative Medicine , University of Leipzig , Leipzig , Germany ;
5
e Centre Hépato-Biliaire , Hôpital Paul Brousse, Université Paris Sud , Villejuif , France.

Abstract

OBJECTIVE:

We investigated whether microRNA signatures in whole blood samples are associated with acute cellular rejection (ACR) after liver transplantation.

MATERIALS AND METHODS:

Blood samples were collected using Paxgene technology and analyzed by microarrays and quantitative real-time polymerase chain reaction (qRT-PCR).

RESULTS:

microRNA signatures failed to distinguish between 19 patients with ACR and 16 controls. Let-7b-5p and let-7c were upregulated in a subgroup of patients with ACR during the 6th and 7th postoperative days but failed in an independent validation of 20 patients.

CONCLUSION:

microRNA signatures in whole blood processed by Paxgene technology are not suited for the detection of ACR after liver transplantation.

KEYWORDS:

Allograft; Paxgene; biomarker

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