Format

Send to

Choose Destination
Methods Mol Biol. 2016;1502:203-11. doi: 10.1007/7651_2016_353.

Bioengineered Models of Solid Human Tumors for Cancer Research.

Author information

1
Department of Biomedical Engineering, Columbia University, New York, NY, USA.
2
Laboratory for Stem Cells and Tissue Engineering, Columbia University, New York, NY, USA.
3
Department of Biomedical Engineering, Columbia University, New York, NY, USA. gv2131@columbia.edu.
4
Laboratory for Stem Cells and Tissue Engineering, Columbia University, New York, NY, USA. gv2131@columbia.edu.
5
Laboratory for Stem Cells and Tissue Engineering, Columbia University, 622 west 168th Street, VC12-234, New York, NY, 10032, USA. gv2131@columbia.edu.

Abstract

The lack of controllable in vitro models that can recapitulate the features of solid tumors such as Ewing's sarcoma limits our understanding of the tumor initiation and progression and impedes the development of new therapies. Cancer research still relies of the use of simple cell culture, tumor spheroids, and small animals. Tissue-engineered tumor models are now being grown in vitro to mimic the actual tumors in patients. Recently, we have established a new protocol for bioengineering the Ewing's sarcoma, by infusing tumor cell aggregates into the human bone engineered from the patient's mesenchymal stem cells. The bone niche allows crosstalk between the tumor cells, osteoblasts and supporting cells of the bone, extracellular matrix, and the tissue microenvironment. The bioreactor platform used in these experiments also allows the implementation of physiologically relevant mechanical signals. Here, we describe a method to build an in vitro model of Ewing's sarcoma that mimics the key properties of the native tumor and provides the tissue context and physical regulatory signals.

KEYWORDS:

Bioreactor platform; Cancer research; Ewing’s sarcoma; Human sarcoma; Tumor model

PMID:
27115504
PMCID:
PMC4969218
DOI:
10.1007/7651_2016_353
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center