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Aging (Albany NY). 2016 May;8(5):1049-63. doi: 10.18632/aging.100952.

Ischemic stroke induces gut permeability and enhances bacterial translocation leading to sepsis in aged mice.

Author information

  • 1University of Connecticut Health Center Department of Neuroscience, Farmington, CT 06030, USA.
  • 2University of Michigan Department of Neuroscience, Ann Arbor, MI 48109, USA.
  • 3University of Connecticut Department of Molecular and Cell Biology, Storrs, CT 06269, USA.
  • 4University of Texas Health Science Center at Houston, McGovern Medical School, Houston, TX 77030, USA.

Abstract

Aging is an important risk factor for post-stroke infection, which accounts for a large proportion of stroke-associated mortality. Despite this, studies evaluating post-stroke infection rates in aged animal models are limited. In addition, few studies have assessed gut microbes as a potential source of infection following stroke. Therefore we investigated the effects of age and the role of bacterial translocation from the gut in post-stroke infection in young (8-12 weeks) and aged (18-20 months) C57Bl/6 male mice following transient middle cerebral artery occlusion (MCAO) or sham surgery. Gut permeability was examined and peripheral organs were assessed for the presence of gut-derived bacteria following stroke. Furthermore, sickness parameters and components of innate and adaptive immunity were examined. We found that while stroke induced gut permeability and bacterial translocation in both young and aged mice, only young mice were able to resolve infection. Bacterial species seeding peripheral organs also differed between young (Escherichia) and aged (Enterobacter) mice. Consequently, aged mice developed a septic response marked by persistent and exacerbated hypothermia, weight loss, and immune dysfunction compared to young mice following stroke.

KEYWORDS:

aging; experimental stroke; infection; inflammation; middle cerebral artery occlusion

PMID:
27115295
PMCID:
PMC4931853
DOI:
10.18632/aging.100952
[PubMed - in process]
Free PMC Article
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