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Carbohydr Polym. 2016 Aug 1;146:187-96. doi: 10.1016/j.carbpol.2016.03.043. Epub 2016 Mar 19.

Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.).

Author information

1
Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea, Republic of Korea.
2
Department of Carbohydrates and Cereals, University of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
3
Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
4
Institute of Organic Chemistry and Biochemistry AS CR, Flemingovo sq. 2, 166 28 Prague 6, Czech Republic.
5
Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea, Republic of Korea. Electronic address: yongil382@catholic.ac.kr.

Abstract

A water-soluble polysaccharide JS-MP-1 was isolated from Korean mulberry fruits Oddi (Morus alba L.). Sugar linkage analysis and NMR data confirmed that it is a rhamnogalacturonan type I (RG I) polymer carrying arabinan and arabinogalactan (AG II) side chains. JS-MP-1 reduced dose-dependently the viability of 3T3-L1 pre-adipocyte cells, significantly stimulated the cleavage of caspases 9 and 3 and poly (ADP-ribose) polymerase (PARP) and decreased the ratio of Bcl-2 to Bax expression level that led to mitochondrial dysfunction and apoptosis in pre-adipocyte cells. The apoptotic death was mediated by stimulation of MAPKs (ERK and p38) signalling pathway. These results suggest that JS-MP-1 is able to reduce the number of fat cells and the mass of adipose tissue via inhibition of pre-adipocyte proliferation and thus JS-MP-1 itself or a crude aqueous Oddi extract containing this polysaccharide can be used as functional ingredient of health-beneficial food supplements for the treatment or prevention of obesity disorders.

KEYWORDS:

Apoptosis; Mulberry fruit; Pectic polysaccharide; Pre-adipocytes; Structure

PMID:
27112865
DOI:
10.1016/j.carbpol.2016.03.043
[Indexed for MEDLINE]

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