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Nat Methods. 2016 Jun;13(6):489-92. doi: 10.1038/nmeth.3840. Epub 2016 Apr 25.

irCLIP platform for efficient characterization of protein-RNA interactions.

Author information

1
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.
2
Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
3
Veterans Affairs, Palo Alto Healthcare System, Palo Alto, California, USA.

Abstract

The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce a UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, and nonisotopic method for the detection of protein-RNA interactions using far less material than standard protocols.

PMID:
27111506
PMCID:
PMC5477425
DOI:
10.1038/nmeth.3840
[Indexed for MEDLINE]
Free PMC Article

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