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Microbes Infect. 2016 Sep;18(9):529-35. doi: 10.1016/j.micinf.2016.04.001. Epub 2016 Apr 22.

Brucella abortus-infected B cells induce osteoclastogenesis.

Author information

1
Instituto de Inmunología, Genética y Metabolismo (INIGEM), Hospital de Clínicas "José de San Martín", Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.
2
Instituto de Inmunología, Genética y Metabolismo (INIGEM), Hospital de Clínicas "José de San Martín", Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina. Electronic address: mdelpino@ffyb.uba.ar.

Abstract

Brucella abortus is an intracellular bacterium that establishes lifelong infections in livestock and humans although the mechanisms of its chronicity are poorly understood. Activated B cells have long lifespan and B. abortus infection activates B cells. Our results indicate that the direct infection of B cells with B. abortus induced matrix metalloproteinase-9 (MMP-9), receptor activator for NF κB ligand (RANKL), tumor necrosis factor (TNF)-α and interleukin (IL)-6 secretion. In addition, supernatants from B. abortus-infected B cells induced bone marrow-derived monocytes to undergo osteoclastogenesis. Using osteoprotegerin, RANKL's decoy receptor, we determined that RANKL is involved in osteoclastogenesis induced by supernatants from B. abortus-infected B cells. The results presented here shed light on how the interactions of B. abortus with B cells may have a role in the pathogenesis of brucellar osteoarticular disease.

KEYWORDS:

B cells; Brucella abortus; Osteoclastogenesis

PMID:
27109230
DOI:
10.1016/j.micinf.2016.04.001
[Indexed for MEDLINE]

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