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Nanotoxicology. 2016 Oct;10(8):1136-43. doi: 10.1080/17435390.2016.1181806. Epub 2016 May 16.

Evaluation of polylactic acid nanoparticles safety using Drosophila model.

Author information

1
a Institut de Biologie et Chimie des Protéines, Fédération de Recherche 3302, SFR BioSciences (Unité Mixte de Service 3444/US8) Gerland-Lyon Sud, Université de Lyon 1 , Lyon , France .
2
b Laboratoire de Biologie Tissulaire et d'Ingénierie Thérapeutique , Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5305 , Lyon , France , and.
3
c Infections Virales et Pathologie Comparée, Institut National De La Recherche Agronomique, Université Claude Bernard Lyon1, Ecole Pratique des Hautes Etudes, Unité Mixte De Recherche 754 , Lyon , France.

Erratum in

Abstract

Cytotoxicity of nanoparticles and their sub-lethal effect on cell behavior and cell fate are a high topic of studies in the nanomaterial field. With an explosion of nanoparticle types (size, shape, polarity, stiffness, composition, etc.), Drosophila has become an attractive animal model for high throughput analysis of these nanocarriers in the drug delivery field with applications in cancer therapy, or simply to generate a fast and complete cytotoxic study of a peculiar nanoparticle. In respect to that, we have conducted an in cellulo study of poly(lactic acid) (PLA) nanoparticle cytotoxicity, and determined that near lethal nanoparticle doses, oxidative stress as well as P53 and ATP pathways may lead to cell cycle arrest at G1, and ultimately to cell death. Neither viability nor the development of Drosophila larvae are affected by the ingestion of PLA nanoparticles at sub-lethal concentrations. Drosophila will be a useful model to study PLA and PLA-modified nanoparticle toxicity, and nanoparticle fate after ingestion.

KEYWORDS:

Cytotoxicity; Drosophila; PLA nanoparticle; oral delivery; sub-lethal concentration

PMID:
27108761
DOI:
10.1080/17435390.2016.1181806
[Indexed for MEDLINE]

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