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Sci Rep. 2016 Apr 25;6:24951. doi: 10.1038/srep24951.

A CRISPR/Cas9 system adapted for gene editing in marine algae.

Author information

1
Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

Abstract

Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.

PMID:
27108533
PMCID:
PMC4842962
DOI:
10.1038/srep24951
[Indexed for MEDLINE]
Free PMC Article

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