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Methods Mol Biol. 2016;1391:13-30. doi: 10.1007/978-1-4939-3332-7_2.

In Vitro Culture and Phytochemical Analysis of Passiflora tenuifila Killip and Passiflora setacea DC (Passifloraceae).

Author information

1
Departamento de Botânica (Centro de Ciências Biológicas), Universidade Federal de Santa Catarina, Campus Reitor João David Ferreira Lima, Florianópolis, SC, CEP: 88040-900, Brazil.
2
Laboratório de Morfogênese e Bioquímica Vegetal (Centro de Ciências Agrárias), Universidade Federal de Santa Catarina, Campus Reitor João David Ferreira Lima, Florianópolis, SC, 88040-900, Brazil.
3
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, Florianópolis, Santa Catarina State, 88040-900, Brazil.
4
Departamento de Botânica (Centro de Ciências Biológicas), Universidade Federal de Santa Catarina, Campus Reitor João David Ferreira Lima, Florianópolis, SC, CEP: 88040-900, Brazil. ana.viana@ufsc.br.

Abstract

We have developed reproducible micropropagation, callus culture, phytochemical, and antioxidant analysis protocols for the wild passion fruit species P. tenuifila, and P. setacea, native to the Brazilian endangered biomes Atlantic Forest, Cerrado, and Caatinga, by using seeds and explants from seedlings and adult plants. Genotype and explant origin-linked differences are visible amongst the Passiflora species concerning callus production, total phenolics, and antioxidant activity. The protocols developed for screening phytochemicals and antioxidants in P. tenuifila and P. setacea callus extracts have shown their potential for phenolic production and antioxidant activity. The high level of phenolic compounds seems to account for the antioxidant activity of methanolic extracts of P. tenuifila derived from 45-day-old immature seed callus. The methanolic extracts of callus derived from P. setacea seedling leaf node and cotyledonary node explants have shown the highest antioxidant activity despite their lower content of phenolics, as compared to cotyledon callus extracts. The optimized micropropagation and callus culture protocols have great potential to use cell culture techniques for further vegetative propagation, in vitro germplasm conservation, and secondary metabolite production using biotic and abiotic elicitors.

KEYWORDS:

Antioxidant activity; Callus; Micropropagation; Passiflora setacea; Passiflora tenuifila; Total phenolics

PMID:
27108307
DOI:
10.1007/978-1-4939-3332-7_2
[Indexed for MEDLINE]

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