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Bioresour Technol. 2016 Jul;212:254-261. doi: 10.1016/j.biortech.2016.04.059. Epub 2016 Apr 21.

Effects of light-emitting diodes (LEDs) on the accumulation of lipid content using a two-phase culture process with three microalgae.

Author information

1
Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
2
Busan Techno-park Marine Bio-industry Development Center (MBDC), Busan 46048, Republic of Korea.
3
Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea. Electronic address: skkim@pknu.ac.kr.

Abstract

Conditions of light-emitting diode (LED) wavelength, light intensity, nitrate concentration, and time of exposure to green LED light stress in a two-phase culture were optimized for lipid production with three species of microalgae. Among the three microalgae, Nannochloropsis oculata showed the highest specific growth rate (μmax) of 0.037h(-1) and the lowest saturation constant (Ks) of 1.32mg/L. The highest lipid contents of the three microalgae in the second phase under green LED light stress were 52.0% (w/w) for Nannochloropsis salina at 2days, 53.0% (w/w) for Nannochloropsis oceanica at 2days, and 56.0% for N. oculata at 2days. Fatty acid analysis of the microalgae showed that 85-87% (w/w) of total fatty acids from N. salina, N. oceanica, and N. oculata consisted of palmitic acid (C16:0) and oleic acid (C18:1).

KEYWORDS:

Fatty acid; Light-emitting diodes (LEDs); Lipid content; Microalgae; Two-phase culture

PMID:
27107342
DOI:
10.1016/j.biortech.2016.04.059
[Indexed for MEDLINE]

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