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Nucleic Acids Res. 2016 Jun 2;44(10):4835-45. doi: 10.1093/nar/gkw291. Epub 2016 Apr 21.

Do nucleic acids moonlight as molecular chaperones?

Author information

1
Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI 48109, USA.
2
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI 48109, USA.
3
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
4
Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI 48109, USA Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI 48109, USA jbardwel@umich.edu.

Abstract

Organisms use molecular chaperones to combat the unfolding and aggregation of proteins. While protein chaperones have been widely studied, here we demonstrate that DNA and RNA exhibit potent chaperone activity in vitro Nucleic acids suppress the aggregation of classic chaperone substrates up to 300-fold more effectively than the protein chaperone GroEL. Additionally, RNA cooperates with the DnaK chaperone system to refold purified luciferase. Our findings reveal a possible new role for nucleic acids within the cell: that nucleic acids directly participate in maintaining proteostasis by preventing protein aggregation.

PMID:
27105849
PMCID:
PMC4889950
DOI:
10.1093/nar/gkw291
[Indexed for MEDLINE]
Free PMC Article

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