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J Intercult Ethnopharmacol. 2016 Mar 24;5(2):186-90. doi: 10.5455/jice.20160320022623. eCollection 2016 Mar-Apr.

Antibacterial efficiency of the Sudanese Roselle (Hibiscus sabdariffa L.), a famous beverage from Sudanese folk medicine.

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Department of Laboratory Sciences, College of Sciences and Arts at Al-Rass, Qassim University, Saudi Arabia.



Hibiscus sabdariffa L. is a plant native to tropical Africa and intensively cultivated in Sudan. Its calyces are widely consumed with many uses in Sudanese folk medicine.


The dried calyces of H. sabdariffa were subjected to soak in 80% v/v methanol to get the methanolic extract, which was tested against five Gram-negative and three Gram-positive referenced bacterial strains using disc diffusion method. Selected bioactive phytochemical compounds were also investigated using qualitative methods.


The results of the antibacterial test indicate that the methanol extract of H. sabdariffa calyces contained effective antibacterial agent(s), revealed a considerable zone of inhibition against all tested Gram-negative and Gram-positive bacteria, and it was a competitor to gentamicin and greatly higher than penicillin which showed weak or no effect.


The results of current investigation support the folk medicine application of this plant against different microbial ailments and suggest it as a promising source for new antibacterial agents.


Antibacterial; Hibiscus sabdariffa; disk diffusion; methanol extract; phytochemical

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