Format

Send to

Choose Destination
J Neurosci Methods. 2016 Jul 15;267:141-9. doi: 10.1016/j.jneumeth.2016.04.012. Epub 2016 Apr 19.

Intact skull chronic windows for mesoscopic wide-field imaging in awake mice.

Author information

1
Department of Psychiatry, University of British Columbia, Vancouver, BC, Canada.
2
Department of Psychiatry, University of British Columbia, Vancouver, BC, Canada; Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China.
3
Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China. Electronic address: ac@ccmu.edu.cn.
4
Department of Psychiatry, University of British Columbia, Vancouver, BC, Canada. Electronic address: thmurphy@mail.ubc.ca.

Abstract

BACKGROUND:

Craniotomy-based window implants are commonly used for microscopic imaging, in head-fixed rodents, however their field of view is typically small and incompatible with mesoscopic functional mapping of cortex.

NEW METHOD:

We describe a reproducible and simple procedure for chronic through-bone wide-field imaging in awake head-fixed mice providing stable optical access for chronic imaging over large areas of the cortex for months.

RESULTS:

The preparation is produced by applying clear-drying dental cement to the intact mouse skull, followed by a glass coverslip to create a partially transparent imaging surface. Surgery time takes about 30min. A single set-screw provides a stable means of attachment (in relation to the measured lateral and axial resolution) for mesoscale assessment without obscuring the cortical field of view.

COMPARISON WITH EXISTING METHODS:

We demonstrate the utility of this method by showing seed-pixel functional connectivity maps generated from spontaneous cortical activity of GCAMP6 signals in both awake and anesthetized mice in longitudinal studies of up to 2 months in duration.

CONCLUSIONS:

We propose that the intact skull preparation described here may be used for most longitudinal studies that do not require micron scale resolution and where cortical neural or vascular signals are recorded with intrinsic sensors or in transgenic mice expressing genetically encoded sensors of activity.

KEYWORDS:

GCaMP; Head-fixed; Optogenetics

PMID:
27102043
PMCID:
PMC5075450
DOI:
10.1016/j.jneumeth.2016.04.012
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center