Send to

Choose Destination
Neurochem Res. 2016 Aug;41(8):1857-67. doi: 10.1007/s11064-016-1911-3. Epub 2016 Apr 21.

Astrocytes Grown in Alvetex(®) Three Dimensional Scaffolds Retain a Non-reactive Phenotype.

Author information

School of Pharmacy, Faculty of Life Sciences, University of Bradford, Richmond Road, Bradford, BD7 1DP, UK.
Reading School of Pharmacy, University of Reading, Whiteknights, Reading, RG6 6UB, UK.
Neurodegeneration and Neurologic Diseases, Pfizer Neuroscience Research Unit, 700 Main Street, Kendall Square, Cambridge, MA, 02149, USA.
School of Pharmacy, Faculty of Life Sciences, University of Bradford, Richmond Road, Bradford, BD7 1DP, UK.


Protocols which permit the extraction of primary astrocytes from either embryonic or postnatal mice are well established however astrocytes in culture are different to those in the mature CNS. Three dimensional (3D) cultures, using a variety of scaffolds may enable better phenotypic properties to be developed in culture. We present data from embryonic (E15) and postnatal (P4) murine primary cortical astrocytes grown on coated coverslips or a 3D polystyrene scaffold, Alvetex. Growth of both embryonic and postnatal primary astrocytes in the 3D scaffold changed astrocyte morphology to a mature, protoplasmic phenotype. Embryonic-derived astrocytes in 3D expressed markers of mature astrocytes, namely the glutamate transporter GLT-1 with low levels of the chondroitin sulphate proteoglycans, NG2 and SMC3. Embryonic astrocytes derived in 3D show lower levels of markers of reactive astrocytes, namely GFAP and mRNA levels of LCN2, PTX3, Serpina3n and Cx43. Postnatal-derived astrocytes show few protein changes between 2D and 3D conditions. Our data shows that Alvetex is a suitable scaffold for growth of astrocytes, and with appropriate choice of cells allows the maintenance of astrocytes with the properties of mature cells and a non-reactive phenotype.


Astrogliosis; Cell culture; EAAT2; GFAP; GLT-1; Glia; Scaffold

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center