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Dev Cell. 2016 Apr 18;37(2):148-61. doi: 10.1016/j.devcel.2016.03.013.

Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation.

Author information

1
Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka 415-0025, Japan. Electronic address: ogura@cdb.riken.jp.
2
Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka 415-0025, Japan.

Abstract

During neurulation of chordate ascidians, the 11th mitotic division within the epidermal layer shows a posterior-to-anterior wave that is precisely coordinated with the unidirectional progression of the morphogenetic movement. Here we show that the first sign of this patterned mitosis is an asynchronous anterior-to-posterior S-phase length and that mitotic synchrony is reestablished by a compensatory asynchronous G2-phase length. Live imaging combined with genetic experiments demonstrated that compensatory G2-phase regulation requires transcriptional activation of the G2/M regulator cdc25 by the patterning genes GATA and AP-2. The downregulation of GATA and AP-2 at the onset of neurulation leads to loss of compensatory G2-phase regulation and promotes the transition to patterned mitosis. We propose that such developmentally regulated cell-cycle compensation provides an abrupt switch to spatially patterned mitosis in order to achieve the coordination between mitotic timing and morphogenesis.

PMID:
27093084
DOI:
10.1016/j.devcel.2016.03.013
[Indexed for MEDLINE]
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