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Nanomedicine (Lond). 2016 May;11(9):1093-105. doi: 10.2217/nnm.16.20. Epub 2016 Apr 13.

Nanoscale bioactive glass activates osteoclastic differentiation of RAW 264.7 cells.

Author information

1
Department of Materials Science & Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany.
2
Department of Gynaecology & Obstetrics, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University-Clinic Erlangen, Universitätsstraße 21-23, 91054 Erlangen, Germany.
3
Institute for Chemical & Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland.
4
Clinic of Preventive Dentistry, Periodontology & Cariology, University of Zurich, Center of Dental Medicine, Plattenstraße 11, 8032 Zurich, Switzerland.
5
Department of Transfusion Medicine & Haemostaseology, University-Clinic Erlangen, Krankenhausstraße 12, 91054 Erlangen, Germany.

Abstract

BACKGROUND:

There is limited knowledge regarding differentiation of osteoclasts in the presence of nanoscale bioactive glass (nBG). This investigation examined increasing concentrations of 45S5 nBG and their influence on osteoclast differentiation.

MATERIALS & METHODS:

Different concentrations of 45S5 nBG were cultured up to 14 days with the murine RAW264.7 cell line and human primary monocytes cultured with M-CSF and RANKL.

RESULTS:

Culturing cells for 14 days with 500 μg/ml nBG showed a viability of 100%; however DNA synthesis was reduced, supporting differentiation into osteoclast-like cells. Using RAW cells, activation of nine genes, including cell fusion genes, occurred in an nBG concentration dependent manner. Low concentrations of nBG increased expression of genes involved in commitment to cell fusion, whereas high concentrations increased gene expression supporting osteoclast-like differentiation.

CONCLUSION:

nBG enhances both RAW264.7 and human osteoclast differentiation. nBG controlled gene expression in a concentration dependent manner could reflect normal regulation during bone growth.

KEYWORDS:

bioactive glass; biomaterials; bone tissue engineering; nanoparticles; osteoclasts

PMID:
27092984
DOI:
10.2217/nnm.16.20
[Indexed for MEDLINE]
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