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Int J Mol Sci. 2016 Apr 15;17(4):568. doi: 10.3390/ijms17040568.

Prognostic Value of MicroRNAs in Preoperative Treated Rectal Cancer.

Author information

1
Department of General, Visceral, and Pediatric Surgery, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. azadeh.azizian@med.uni-goettingen.de.
2
Department of General, Visceral, and Pediatric Surgery, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. ingo-epping@web.de.
3
Department of Medical Statistics, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. frank.kramer@med.uni-goettingen.de.
4
Department of General, Visceral, and Pediatric Surgery, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. jo.peter@chirurgie-goettingen.de.
5
Department of General, Visceral, and Pediatric Surgery, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. markus.bernhardt@med.uni-goettingen.de.
6
Department of Pathology, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. j.kitz@med.uni-goettingen.de.
7
Department of Developmental Biochemistry, University of Göttingen, Göttingen 37075, Germany. gsalina@gwdg.de.
8
Medical Practice Radiotherapy München, Burgstraße 7, München 80331, Germany. hendrik.wolff@med.uni-goettingen.de.
9
Department of General, Visceral, and Pediatric Surgery, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. marian.grade@med.uni-goettingen.de.
10
Department of Medical Statistics, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. tim.beissbarth@med.uni-goettingen.de.
11
Department of General, Visceral, and Pediatric Surgery, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. mghadim@uni-goettingen.de.
12
Department of General, Visceral, and Pediatric Surgery, University Medical Center Göttingen, Robert-Koch-Str. 40, Göttingen 37075, Germany. jochen.gaedcke@med.uni-goettingen.de.

Abstract

BACKGROUND:

Patients with locally advanced rectal cancer are treated with preoperative chemoradiotherapy followed by surgical resection. Despite similar clinical parameters (uT2-3, uN+) and standard therapy, patients' prognoses differ widely. A possible prediction of prognosis through microRNAs as biomarkers out of treatment-naïve biopsies would allow individualized therapy options.

METHODS:

Microarray analysis of 45 microdissected preoperative biopsies from patients with rectal cancer was performed to identify potential microRNAs to predict overall survival, disease-free survival, cancer-specific survival, distant-metastasis-free survival, tumor regression grade, or nodal stage. Quantitative real-time polymerase chain reaction (qPCR) was performed on an independent set of 147 rectal cancer patients to validate relevant miRNAs.

RESULTS:

In the microarray screen, 14 microRNAs were significantly correlated to overall survival. Five microRNAs were included from previous work. Finally, 19 miRNAs were evaluated by qPCR. miR-515-5p, miR-573, miR-579 and miR-802 demonstrated significant correlation with overall survival and cancer-specific survival (p < 0.05). miR-573 was also significantly correlated with the tumor regression grade after preoperative chemoradiotherapy. miR-133b showed a significant correlation with distant-metastasis-free survival. miR-146b expression levels showed a significant correlation with nodal stage.

CONCLUSION:

Specific microRNAs can be used as biomarkers to predict prognosis of patients with rectal cancer and possibly stratify patients' therapy if validated in a prospective study.

KEYWORDS:

biomarkers; chemoradiotherapy; miRNA; prognosis; rectal cancer; tumor regression grade

PMID:
27092493
PMCID:
PMC4849024
DOI:
10.3390/ijms17040568
[Indexed for MEDLINE]
Free PMC Article

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