Format

Send to

Choose Destination
Clin Infect Dis. 2016 Aug 1;63(3):380-6. doi: 10.1093/cid/ciw242. Epub 2016 Apr 18.

Implementation of Nationwide Real-time Whole-genome Sequencing to Enhance Listeriosis Outbreak Detection and Investigation.

Author information

1
Centers for Disease Control and Prevention, Atlanta, Georgia.
2
Food and Drug Administration, College Park, Maryland.
3
US Department of Agriculture, Food Safety and Inspection Service, Athens, Georgia.
4
US Department of Agriculture, Food Safety and Inspection Service, Washington D.C.
5
Association of Public Health Laboratories, Silver Spring, Maryland.
6
Applied Maths, Sint-Martens-Latem, Belgium.
7
National Institute for Biotechnology Information, National Institutes of Health, Bethesda, Maryland.

Abstract

Listeria monocytogenes (Lm) causes severe foodborne illness (listeriosis). Previous molecular subtyping methods, such as pulsed-field gel electrophoresis (PFGE), were critical in detecting outbreaks that led to food safety improvements and declining incidence, but PFGE provides limited genetic resolution. A multiagency collaboration began performing real-time, whole-genome sequencing (WGS) on all US Lm isolates from patients, food, and the environment in September 2013, posting sequencing data into a public repository. Compared with the year before the project began, WGS, combined with epidemiologic and product trace-back data, detected more listeriosis clusters and solved more outbreaks (2 outbreaks in pre-WGS year, 5 in WGS year 1, and 9 in year 2). Whole-genome multilocus sequence typing and single nucleotide polymorphism analyses provided equivalent phylogenetic relationships relevant to investigations; results were most useful when interpreted in context of epidemiological data. WGS has transformed listeriosis outbreak surveillance and is being implemented for other foodborne pathogens.

KEYWORDS:

DNA sequencing; Listeria monocytogenes; foodborne diseases; outbreaks

PMID:
27090985
PMCID:
PMC4946012
DOI:
10.1093/cid/ciw242
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center