Format

Send to

Choose Destination
J Microbiol Biotechnol. 2016 Jul 28;26(7):1216-23. doi: 10.4014/jmb.1601.01033.

Purification and Identification of Antioxidant Peptides from Enzymatic Hydrolysate of Spirulina platensis.

Yu J1,2, Hu Y1,2, Xue M3, Dun Y2, Li S2, Peng N2, Liang Y2,4, Zhao S2,4.

Author information

1
Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, P.R. China.
2
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China.
3
Beihai Shengbada Biotech Co., Ltd, Beihai 536000, P.R. China.
4
Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430068, P.R. China.

Abstract

The aim of this study was to isolate antioxidant peptides from an enzymatic hydrolysate of Spirulina platensis. A novel antioxidant peptide was obtained by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography, with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay used to measure the antioxidant activity, and the sequence was determined to be Pro-Asn-Asn (343.15 Da) by electrospray ionization tandem mass spectrometry. This peptide was synthesized to confirm its antioxidant properties, and it exhibited 81.44 ± 0.43% DPPH scavenging activity at 100 µg/ml, which was similar to that of glutathione (82.63 ± 0.56%). Furthermore, the superoxide anion and hydroxyl free-radical scavenging activities and the SOD activity of the peptide were 47.84 ± 0.49%, 54.01 ± 0.82%, and 12.55 ± 0.75%, respectively, at 10 mg/ml. These results indicate that S. platensis is a good source of antioxidant peptides, and that its hydrolysate may have important applications in the pharmaceutical and food industries.

KEYWORDS:

Antioxidant peptide; Spirulina platensis; free-radical scavenging activity; mass spectrometry; purification

PMID:
27090190
DOI:
10.4014/jmb.1601.01033
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for The Korean Society for Microbiology and Biotechnology.
Loading ...
Support Center