Format

Send to

Choose Destination
Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.

Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.

Author information

1
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.
2
Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA.
3
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA.
4
Department of Cell Biology &Physiology, University of North Carolina, Chapel Hill, North Carolina, USA.

Abstract

We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.

PMID:
27089030
PMCID:
PMC4888063
DOI:
10.1038/nchembio.2068
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center