Format

Send to

Choose Destination
Nat Methods. 2016 Jun;13(6):501-4. doi: 10.1038/nmeth.3836. Epub 2016 Apr 18.

siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.

Author information

1
Department of Cell Biology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
2
Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
3
Lambert Instruments B.V., Groningen, the Netherlands.
4
Swammerdam Institute for Life Sciences, Section of Molecular Cytology, University of Amsterdam, Amsterdam, the Netherlands.
5
Van Leeuwenhoek Centre for Advanced Microscopy, Amsterdam, the Netherlands.

Abstract

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.

PMID:
27088314
DOI:
10.1038/nmeth.3836
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center