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J Agric Food Chem. 2016 May 11;64(18):3564-73. doi: 10.1021/acs.jafc.6b00425. Epub 2016 Apr 26.

Antiallergic Activity of Ethanol Extracts of Arctium lappa L. Undried Roots and Its Active Compound, Oleamide, in Regulating FcεRI-Mediated and MAPK Signaling in RBL-2H3 Cells.

Author information

1
School of Life Sciences and Biotechnology, Kyungpook National University , Daegu 41566, Republic of Korea.
2
Cardiovascular and Metabolic Disease Center and Department of Health Sciences and Technology, Inje University , Busan 47392, Republic of Korea.
3
Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University , Yongin-si 17104, Republic of Korea.
4
Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd. , Seongnam-si 13605, Republic of Korea.
5
Department of Herbal Medicine Resource, Kangwon National University , Samcheok 25913, Republic of Korea.

Abstract

The antiallergic potential of Arctium lappa L. was investigated in Sprague-Dawley rats, ICR mice, and RBL-2H3 cells. Ethanol extract (90%) of A. lappa (ALE, 100 μg/mL) inhibited the degranulation rate by 52.9%, determined by the level of β-hexosaminidase. ALE suppressed passive cutaneous anaphylaxis (PCA) in rats and attenuated anaphylaxis and histamine release in mice. To identify the active compound of ALE, we subsequently fractionated and determined the level of β-hexosaminidase in all subfractions. Oleamide was identified as an active compound of ALE, which attenuated the secretion of histamine and the production of tumor necrosis factor (TNF)-α and interleukin-4 (IL-4) in cells treated with compound 48/80 or A23187/phorbol myristate acetate (PMA). Oleamide suppressed FcεRI-tyrosine kinase Lyn-mediated pathway, c-Jun N-terminal kinases (JNK/SAPK), and p38 mitogen-activated protein kinases (p38-MAPKs). These results showed that ALE and oleamide attenuated allergic reactions and should serve as a platform to search for compounds with antiallergic activity.

KEYWORDS:

Arctium lappa L; Lyn; RBL-2H3 cell; antiallergic; oleamide

PMID:
27087645
DOI:
10.1021/acs.jafc.6b00425
[Indexed for MEDLINE]

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