Format

Send to

Choose Destination
Asian Pac J Trop Med. 2016 Apr;9(4):357-365. doi: 10.1016/j.apjtm.2016.03.017. Epub 2016 Mar 10.

Fruits extracts of Hovenia dulcis Thunb. suppresses lipopolysaccharide-stimulated inflammatory responses through nuclear factor-kappaB pathway in Raw 264.7 cells.

Author information

1
Department of Acupoint, College of Korean Medicine, Dongguk University, Siksa-dong, Ilsan, Goyang-si, Gyeonggi-do 10326, Republic of Korea.
2
Department of Prescription, College of Korean Medicine, Dongguk University, Siksa-dong, Ilsan, Goyang-si, Gyeonggi-do 10326, Republic of Korea.
3
Department of Diagnostics, College of Korean Medicine, Dongguk University, Siksa-dong, Ilsan, Goyang-si, Gyeonggi-do 10326, Republic of Korea.
4
Department of Diagnostics, College of Korean Medicine, Dongguk University, Siksa-dong, Ilsan, Goyang-si, Gyeonggi-do 10326, Republic of Korea. Electronic address: hyuckkim@dongguk.ac.kr.
5
Department of Pathology, College of Korean Medicine, Dongguk University, Siksa-dong, Ilsan, Goyang-si, Gyeonggi-do 10326, Republic of Korea. Electronic address: herbqueen@dongguk.ac.kr.

Abstract

OBJECTIVE:

To investigate the anti-inflammatory effects and the action mechanism of the fruits of Hovenia dulcis (H. dulcis) in lipopolysaccharide (LPS)-induced mouse macrophage Raw 264.7 cells.

METHODS:

The extract of H. dulcis fruits (EHDF) were extracted with 70% ethanol. Mouse macrophages were treated with different concentrations of EHDF in the presence and absence of LPS (1 μg/mL). To demonstrate the inflammatory mediators including nitric oxide, inducible nitric oxide synthase and cyclooxygenase (COX)-2 expression levels were analyzed by using in vitro assay systems. COX-derived pro-inflammatory cytokines including interleukin-1β, tumor necrosis factor-α and prostaglandin E2 were determined using ELISA kits. Cell viability, heme oxygenase-1 expression, nuclear factor-kappaB and nuclear factor E2-related factors 2 translocation were also investigated.

RESULTS:

EHDF potently inhibited the LPS-stimulated nitric oxide, inducible nitric oxide synthase, COX-2, interleukin-1β and tumor necrosis factor-α expression in a dose-dependent manner. EHDF suppressed the phosphorylation of inhibited kappaB-alpha and p65 nuclear translocation. Treatment of macrophage cells with EHDF alone induced the heme oxygenase-1 and nuclear translocation of nuclear factor E2-related factor 2.

CONCLUSIONS:

These results suggest that the ethanol extract of H. dulcis fruit exerts its anti-inflammatory effects by inhibiting inhibited kappaB-alpha phorylation and nuclear translocation of nuclear factor-kappaB.

KEYWORDS:

Fruits of Hovenia dulcis; Heme oxygenase-1; Inflammation; Macrophage; Nuclear factor-kappaB

PMID:
27086154
DOI:
10.1016/j.apjtm.2016.03.017
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center