Format

Send to

Choose Destination
J Bone Miner Metab. 2017 May;35(3):255-264. doi: 10.1007/s00774-016-0757-8. Epub 2016 Apr 16.

MiR-142-5p promotes bone repair by maintaining osteoblast activity.

Author information

1
Institute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, People's Republic of China.
2
Department of Gynaecology and Obstetrics, The First Hospital Affiliated to Nanjing Medical University, Nanjing, 210029, Jiangsu, People's Republic of China.
3
Department of Orthopedics, Xiangya Hospital of Central South University, 87# Xiangya Road, Changsha, 410008, Hunan, People's Republic of China.
4
Department of Gerontology, The First Hospital Affiliated to Nanjing Medical University, Nanjing, 210029, Jiangsu, People's Republic of China. cphh@sohu.com.
5
Department of Endocrinology and Metabolism, The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, People's Republic of China. chaochen2005@163.com.

Abstract

MicroRNAs play important roles in regulating bone regeneration and remodeling. However, the pathophysiological roles of microRNAs in bone repair remain unclear. Here we identify a significant upregulation of miR-142-5p correlated with active osteoblastogenesis during the bone healing process. In vitro, miR-142-5p promoted osteoblast activity and matrix mineralization by targeting the gene encoding WW-domain-containing E3 ubiquitin protein ligase 1. We also found that the expression of miR-142-5p in the callus of aged mice was lower than that in the callus of young mice and directly correlated with the age-related delay in bone healing. Furthermore, treatment with agomir-142-5p in the fracture areas stimulated osteoblast activity which repaired the bone fractures in aged mice. Thus, our study revealed that miR-142-5p plays a crucial role in healing fractures by maintaining osteoblast activity, and provided a new molecular target therapeutic strategy for bone healing.

KEYWORDS:

Bone fracture; MicroRNA; Osteoblastogenesis; Wwp1

PMID:
27085967
DOI:
10.1007/s00774-016-0757-8
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center