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Plant Cell Physiol. 2016 May;57(5):961-75. doi: 10.1093/pcp/pcw067. Epub 2016 Apr 15.

Jasmonate-Responsive ERF Transcription Factors Regulate Steroidal Glycoalkaloid Biosynthesis in Tomato.

Author information

1
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101 Japan.
2
Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, Tsu, 514-2392 Japan.
3
Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571 Japan.
4
RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045 Japan.
5
Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro-ku, 152-8551 Japan.
6
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8577 Japan.
7
RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045 Japan Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, 260-8675 Japan.
8
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101 Japan t-shouji@bs.naist.jp.

Abstract

Steroidal glycoalkaloids (SGAs) are cholesterol-derived specialized metabolites produced in species of the Solanaceae. Here, we report that a group of jasmonate-responsive transcription factors of the ETHYLENE RESPONSE FACTOR (ERF) family (JREs) are close homologs of alkaloid regulators in Cathranthus roseus and tobacco, and regulate production of SGAs in tomato. In transgenic tomato, overexpression and dominant suppression of JRE genes caused drastic changes in SGA accumulation and in the expression of genes for metabolic enzymes involved in the multistep pathway leading to SGA biosynthesis, including the upstream mevalonate pathway. Transactivation and DNA-protein binding assays demonstrate that JRE4 activates the transcription of SGA biosynthetic genes by binding to GCC box-like elements in their promoters. These JRE-binding elements occur at significantly higher frequencies in proximal promoter regions of the genes regulated by JRE genes, supporting the conclusion that JREs mediate transcriptional co-ordination of a series of metabolic genes involved in SGA biosynthesis.

KEYWORDS:

Jasmonates; Steroidal glycoalkaloids; Tomato; Transcription factors

PMID:
27084593
DOI:
10.1093/pcp/pcw067
[Indexed for MEDLINE]

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