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FEMS Microbiol Ecol. 2016 Jun;92(6):fiw075. doi: 10.1093/femsec/fiw075. Epub 2016 Apr 15.

Characterization of tet(Y)-carrying LowGC plasmids exogenously captured from cow manure at a conventional dairy farm.

Author information

1
Biology Centre of the Czech Academy of Sciences, Institute of Soil Biology, Na Sádkách 7, 370 05 České Budějovice, Czech Republic.
2
Biology Centre of the Czech Academy of Sciences, Institute of Soil Biology, Na Sádkách 7, 370 05 České Budějovice, Czech Republic chrudimsky@upb.cas.cz.
3
Biology Centre of the Czech Academy of Sciences, Institute of Parasitology, Branišovská 31, 370 05 České Budějovice, Czech Republic Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic.
4
Department of Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants (JKI), Messeweg 11/12, 38104 Braunschweig, Germany.

Abstract

Manure from dairy farms has been shown to contain diverse tetracycline resistance genes that are transferable to soil. Here, we focus on conjugative plasmids that may spread tetracycline resistance at a conventional dairy farm. We performed exogenous plasmid isolation from cattle feces using chlortetracycline for transconjugant selection. The transconjugants obtained harbored LowGC-type plasmids and tet(Y). A representative plasmid (pFK2-7) was fully sequenced and this was compared with previously described LowGC plasmids from piggery manure-treated soil and a GenBank record from Acinetobacter nosocomialis that we also identified as a LowGC plasmid. The pFK2-7 plasmid had the conservative backbone typical of LowGC plasmids, though this region was interrupted with an insert containing the tet(Y)-tet(R) tetracycline resistance genes and the strA-strB streptomycin resistance genes. Despite Acinetobacter populations being considered natural hosts of LowGC plasmids, these plasmids were not found in three Acinetobacter isolates from the study farm. The isolates harbored tet(Y)-tet(R) genes in identical genetic surroundings as pFK2-7, however, suggesting genetic exchange between Acinetobacter and LowGC plasmids. Abundance of LowGC plasmids and tet(Y) was correlated in manure and soil samples from the farm, indicating that LowGC plasmids may be involved in the spread of tet(Y) in the environment.

KEYWORDS:

Acinetobacter; LowGC plasmids; cattle manure; horizontal gene transfer; tet(Y); tetracycline resistance

PMID:
27083193
DOI:
10.1093/femsec/fiw075
[Indexed for MEDLINE]

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