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Virology. 2016 Jul;494:56-66. doi: 10.1016/j.virol.2016.03.028. Epub 2016 Apr 12.

Two asian jumbo phages, ϕRSL2 and ϕRSF1, infect Ralstonia solanacearum and show common features of ϕKZ-related phages.

Author information

1
Plant Research Laboratory, National Center for Genetic Engineering and Biotechnology, NSTDA, Pathum Thani 12120, Thailand; Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand; Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand.
2
Bioinformatics Center, Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
3
Department of Microbiology and Botany, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
4
Plant Research Laboratory, National Center for Genetic Engineering and Biotechnology, NSTDA, Pathum Thani 12120, Thailand.
5
Department of Plant Pathology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
6
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
7
Bioinformatics Center, Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. Electronic address: ogata@kuicr.kyoto-u.ac.jp.
8
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan. Electronic address: tayamad@hiroshima-u.ac.jp.

Abstract

Jumbo phages infecting Ralstonia solanacearum were isolated in Thailand (ϕRSL2) and Japan (ϕRSF1). They were similar regarding virion morphology, genomic arrangement, and host range. Phylogenetic and proteomic tree analyses demonstrate that the ϕRSL2 and ϕRSF1 belong to a group of evolutionary related phages, including Pseudomonas phages ϕKZ, 201ϕ2-1 and all previously described ϕKZ-related phages. Despite conserved genomic co-linearity between the ϕRSL2 and ϕRSF1, they differ in protein separation patterns. A major difference was seen in the detection of virion-associated-RNA polymerase subunits. All β- and β'-subunits were detected in ϕRSF1, but one β'-subunit was undetected in ϕRSL2. Furthermore, ϕRSF1 infected host cells faster (latent period: 60 and 150min for ϕRSF1 and ϕRSL2, respectively) and more efficiently than ϕRSL2. Therefore, the difference in virion-associated-RNA polymerase may affect infection efficiency. Finally, we show that ϕRSF1 is able to inhibit bacterial wilt progression in tomato plants.

KEYWORDS:

Genomic analysis; Jumbo phages; Ralstonia solanacearum; Virion-associated-RNA polymerase; ϕKZ-like phages

PMID:
27081857
DOI:
10.1016/j.virol.2016.03.028
[Indexed for MEDLINE]
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