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Neurol Res. 2016 Jan;38(1):75-9. doi: 10.1080/01616412.2015.1135558. Epub 2016 Feb 18.

Normobaric hyperoxia retards the evolution of ischemic brain tissue toward infarction in a rat model of transient focal cerebral ischemia.

Xu J1,2,3, Zhang Y1,2, Liang Z1,4, Wang T1,2, Li W2,5, Ren L2,4, Huang S3, Liu W1,2,5.

Author information

1
a The Central Laboratory, Shenzhen 2nd People's Hospital , Shenzhen University 1st Affiliated Hospital , Shenzhen , China.
2
b Shenzhen Key Laboratory of Neurosurgery, Shenzhen 2nd People's Hospital , Shenzhen University 1st Affiliated Hospital , Shenzhen , China.
3
e Department of Anesthesiology, Shenzhen 2nd People's Hospital , Shenzhen University 1st Affiliated Hospital , Shenzhen , China.
4
d Department of Neurology, Shenzhen 2nd People's Hospital , Shenzhen University 1st Affiliated Hospital , Shenzhen , China.
5
c Department of Neurosurgery, Shenzhen 2nd People's Hospital , Shenzhen University 1st Affiliated Hospital , Shenzhen , China.

Abstract

OBJECTIVES:

Oxygen therapy has been long considered a logical therapy for ischemic stroke. Our previous studies showed that normobaric hyperoxia (normobaric hyperoxia (NBO), 95% O2 with 5% CO2) treatment during ischemia reduced ischemic neuronal death and cerebromicrovascular injury in animal stroke models. In this study, we studied the effects of NBO on the evolution of ischemic brain tissue to infarction in a rat model of transient focal cerebral ischemia.

METHODS:

Male Sprague-Dawley rats were given NBO (95% O2) or normoxia (21% O2) during 90-min filament occlusion of the middle cerebral artery (MCAO), followed by 3 or 22.5 h of reperfusion. 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to evaluate the longitudinal evolution of tissue infarction. Results: In normoxic rats, MCA-supplied cortical and striatal tissue was infarcted after 90-min MCAO with 22.5 h of reperfusion. NBO-treated rats showed a 61.4% reduction in infarct size and tissue infarction mainly occurred in the ischemic striatum. When infarction was assessed at an earlier time point, i.e. at 3 h of reperfusion, normoxic rats showed significantly smaller but mature infarction (no TTC staining, white color), with the infarction mainly occurring in the striatum. Unexpectedly, NBO-treated rats only showed immature lesion (partially stained by TTC, light white color) in the ischemic striatum, indicating that NBO treatment also retarded the process of neuronal death in the ischemic core. Of note, NBO-preserved striatal tissue underwent infarction after prolonged reperfusion. Conclusions: Our results demonstrate that NBO treatment given during cerebral ischemia retards the evolution of ischemic brain tissue toward infarction and NBO-preserved cortical tissue survives better than NBO-preserved striatal tissue during the phase of reperfusion.

KEYWORDS:

Neuroprotection; Oxygen; Stroke; infarction

PMID:
27078693
DOI:
10.1080/01616412.2015.1135558
[Indexed for MEDLINE]

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