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Nanomedicine (Lond). 2016 May;11(9):1073-91. doi: 10.2217/nnm-2015-0021. Epub 2016 Apr 14.

A 3D vascularized bone remodeling model combining osteoblasts and osteoclasts in a CaP nanoparticle-enriched matrix.

Author information

1
Cell and Tissue Engineering Laboratory, IRCCS Galeazzi Orthopaedic Institute, 20161 Milan, Italy.
2
PhD School in Life Sciences, Department of Biotechnology & Biosciences, University of Milano-Bicocca, 20126 Milan, Italy.
3
Regenerative Medicine Technologies Lab, Ente Ospedaliero Cantonale (EOC), 6900 Lugano, Switzerland.
4
Swiss Institute of Regenerative Medicine (SIRM), 6900 Lugano, Switzerland.
5
Fondazione Cardiocentro Ticino, 6900 Lugano, Switzerland.

Abstract

AIM:

We aimed to establish a 3D vascularized in vitro bone remodeling model.

MATERIALS & METHODS:

Human umbilical endothelial cells (HUVECs), bone marrow mesenchymal stem cells (BMSCs), and osteoblast (OBs) and osteoclast (OCs) precursors were embedded in collagen/fibrin hydrogels enriched with calcium phosphate nanoparticles (CaPn). We assessed vasculogenesis in HUVEC-BMSC coculture, osteogenesis with OBs, osteoclastogenesis with OCs, and, ultimately, cell interplay in tetraculture.

RESULTS:

HUVECs developed a robust microvascular network and BMSCs differentiated into mural cells. Noteworthy, OB and OC differentiation was increased by their reciprocal coculture and by CaPn, and even more by the combination of the tetraculture and CaPn.

CONCLUSION:

We successfully developed a vascularized 3D bone remodeling model, whereby cells interacted and exerted their specific function.

KEYWORDS:

3D model; bone remodeling; vascularization

PMID:
27078586
DOI:
10.2217/nnm-2015-0021
[Indexed for MEDLINE]

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