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Methods Mol Biol. 2016;1404:741-52. doi: 10.1007/978-1-4939-3389-1_48.

The Web-Based DNA Vaccine Database DNAVaxDB and Its Usage for Rational DNA Vaccine Design.

Racz R1,2, He Y3,4,5,6.

Author information

1
College of Pharmacy, University of Michigan, Ann Arbor, MI, 48109, USA.
2
Unit for Laboratory Animal Medicine, University of Michigan Medical School, A317A, Medical School Research Building III, Ann Arbor, MI, 48109, USA.
3
Unit for Laboratory Animal Medicine, University of Michigan Medical School, A317A, Medical School Research Building III, Ann Arbor, MI, 48109, USA. yongqunh@umich.edu.
4
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA. yongqunh@umich.edu.
5
Center for Computational Medicine and Bioinformatics, University of Michigan Medical School, Ann Arbor, MI, 48109, USA. yongqunh@umich.edu.
6
Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI, 48109, USA. yongqunh@umich.edu.

Abstract

A DNA vaccine is a vaccine that uses a mammalian expression vector to express one or more protein antigens and is administered in vivo to induce an adaptive immune response. Since the 1990s, a significant amount of research has been performed on DNA vaccines and the mechanisms behind them. To meet the needs of the DNA vaccine research community, we created DNAVaxDB ( http://www.violinet.org/dnavaxdb ), the first Web-based database and analysis resource of experimentally verified DNA vaccines. All the data in DNAVaxDB, which includes plasmids, antigens, vaccines, and sources, is manually curated and experimentally verified. This chapter goes over the detail of DNAVaxDB system and shows how the DNA vaccine database, combined with the Vaxign vaccine design tool, can be used for rational design of a DNA vaccine against a pathogen, such as Mycobacterium bovis.

KEYWORDS:

DNA vaccine; DNAVaxDB; Data analysis; Database; Plasmid DNA; Protective antigen

PMID:
27076334
DOI:
10.1007/978-1-4939-3389-1_48
[Indexed for MEDLINE]

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