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Methods Mol Biol. 2016;1404:351-64. doi: 10.1007/978-1-4939-3389-1_24.

Generation of Lymphocytic Choriomeningitis Virus Based Vaccine Vectors.

Author information

1
Institute of Immunobiology, Kantonsspital St. Gallen, Rorschacher Str. 95, St. Gallen, 9007, Switzerland.
2
Institute of Immunobiology, Kantonsspital St. Gallen, Rorschacher Str. 95, St. Gallen, 9007, Switzerland. lukas.flatz@gmail.com.

Abstract

Vaccination with a recombinant LCMV based vector expressing tumor-associated or viral antigens is a safe and versatile method to induce an immune response against tumors or viral infections. Here, we describe the generation of recombinant LCMV vectors in which the gene encoding the viral LCMV-GP was substituted with a gene of interest (vaccine antigen). This renders the vaccine vector propagation-incompetent while it preserves the property of eliciting a strong cytotoxic T cell response.

KEYWORDS:

Arenavirus; CTL response; LCMV; Reverse genetics; Viral vaccine vectors

PMID:
27076310
DOI:
10.1007/978-1-4939-3389-1_24
[Indexed for MEDLINE]

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