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Sci Rep. 2016 Apr 14;6:24444. doi: 10.1038/srep24444.

A Fluorescence Approach to Assess the Production of Soluble Microbial Products from Aerobic Granular Sludge Under the Stress of 2,4-Dichlorophenol.

Author information

1
School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
2
School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia.
3
Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

Abstract

In this study, a fluorescence approach was used to evaluate the production of soluble microbial products (SMP) in aerobic granular sludge system under the stress of 2,4-dichlorophenol (2,4-DCP). A combined use of three-dimension excitation emission matrix fluorescence spectroscopy (3D-EEM), Parallel factor analysis (PARAFAC), synchronous fluorescence and two-dimensional correlation spectroscopy (2D-COS) were explored to respect the SMP formation in the exposure of different doses of 2,4-DCP. Data implied that the presence of 2,4-DCP had an obvious inhibition on biological nitrogen removal. According to EEM-PARAFAC, two fluorescent components were derived and represented to the presence of fulvic-like substances and humic-like substances in Component 1 and protein-like substances in Component 2. It was found from synchronous fluorescence that protein-like peak presented slightly higher intensity than that of fulvic-like peak. 2D-COS further revealed that fluorescence change took place sequentially in the following order: protein-like fraction > fulvic-like fraction. The obtained results could provide a potential application of fluorescence spectra in the released SMP assessment in the exposure of toxic compound during wastewater treatment.

PMID:
27075778
PMCID:
PMC4830994
DOI:
10.1038/srep24444
[Indexed for MEDLINE]
Free PMC Article

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