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Anal Bioanal Chem. 2016 Jun;408(16):4267-73. doi: 10.1007/s00216-016-9525-9. Epub 2016 Apr 13.

Metabolomics reveals mycoplasma contamination interferes with the metabolism of PANC-1 cells.

Author information

1
School of Pharmaceutical Sciences, Sun Yat-sen University, 132# Waihuandong Rd, University City of Guangzhou, Guangzhou, 510006, China.
2
Department of Environmental Health Sciences, Yale School of Public Health, Yale University, 60 College Street, New Haven, CT, 06520, USA.
3
Thermo Fisher Scientific, Xin Jinqiao Rd., Shanghai, 201206, China.
4
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, 20892, USA.
5
Department of Biochemistry and Molecular Medicine, UC Davis Medical Center, Sacramento, CA, 95817, USA.
6
School of Pharmaceutical Sciences, Sun Yat-sen University, 132# Waihuandong Rd, University City of Guangzhou, Guangzhou, 510006, China. bihchang@mail.sysu.edu.cn.

Abstract

Mycoplasma contamination is a common problem in cell culture and can alter cellular functions. Since cell metabolism is either directly or indirectly involved in every aspect of cell function, it is important to detect changes to the cellular metabolome after mycoplasma infection. In this study, liquid chromatography mass spectrometry (LC/MS)-based metabolomics was used to investigate the effect of mycoplasma contamination on the cellular metabolism of human pancreatic carcinoma cells (PANC-1). Multivariate analysis demonstrated that mycoplasma contamination induced significant metabolic changes in PANC-1 cells. Twenty-three metabolites were identified and found to be involved in arginine and purine metabolism and energy supply. This study demonstrates that mycoplasma contamination significantly alters cellular metabolite levels, confirming the compelling need for routine checking of cell cultures for mycoplasma contamination, particularly when used for metabolomics studies. Graphical abstract Metabolomics reveals mycoplasma contamination changes the metabolome of PANC-1 cells.

KEYWORDS:

Cell culture; Cellular metabolism; Metabolomics; Mycoplasma contamination; PANC-1 cells

PMID:
27074779
DOI:
10.1007/s00216-016-9525-9
[Indexed for MEDLINE]

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