A coniferyl aldehyde dehydrogenase gene from Pseudomonas sp. strain HR199 enhances the conversion of coniferyl aldehyde by Saccharomyces cerevisiae

Peter Temitope Adeboye; Lisbeth Olsson; Maurizio Bettiga

doi:10.1016/j.biortech.2016.04.003

A coniferyl aldehyde dehydrogenase gene from Pseudomonas sp. strain HR199 enhances the conversion of coniferyl aldehyde by Saccharomyces cerevisiae

Bioresour Technol. 2016 Jul:212:11-19. doi: 10.1016/j.biortech.2016.04.003. Epub 2016 Apr 5.

Authors

Peter Temitope Adeboye¹, Lisbeth Olsson¹, Maurizio Bettiga²

Affiliations

¹ Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
² Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden. Electronic address: maurizio.bettiga@chalmers.se.

PMID: 27070284
DOI: 10.1016/j.biortech.2016.04.003

Abstract

The conversion of coniferyl aldehyde to cinnamic acids by Saccharomyces cerevisiae under aerobic growth conditions was previously observed. Bacteria such as Pseudomonas have been shown to harbor specialized enzymes for converting coniferyl aldehyde but no comparable enzymes have been identified in S. cerevisiae. CALDH from Pseudomonas was expressed in S. cerevisiae. An acetaldehyde dehydrogenase (Ald5) was also hypothesized to be actively involved in the conversion of coniferyl aldehyde under aerobic growth conditions in S. cerevisiae. In a second S. cerevisiae strain, the acetaldehyde dehydrogenase (ALD5) was deleted. A prototrophic control strain was also engineered. The engineered S. cerevisiae strains were cultivated in the presence of 1.1mM coniferyl aldehyde under aerobic condition in bioreactors. The results confirmed that expression of CALDH increased endogenous conversion of coniferyl aldehyde in S. cerevisiae and ALD5 is actively involved with the conversion of coniferyl aldehyde in S. cerevisiae.

Keywords: Coniferyl aldehyde; Lignocellulose conversion; Phenolics conversion; Phenolics tolerance; Saccharomyces cerevisiae.

MeSH terms

Acrolein / analogs & derivatives*
Acrolein / metabolism
Aldehyde Oxidoreductases / genetics*
Aldehyde Oxidoreductases / metabolism
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Organisms, Genetically Modified / metabolism
Pseudomonas / enzymology
Pseudomonas / genetics*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*

Substances

Bacterial Proteins
coniferaldehyde
Acrolein
Aldehyde Oxidoreductases
coniferyl aldehyde dehydrogenase
aldehyde dehydrogenase (NAD(P)+)