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Appl Biochem Biotechnol. 2016 Aug;179(8):1435-44. doi: 10.1007/s12010-016-2075-y. Epub 2016 Apr 11.

Biotransformation of L-tyrosine to Dopamine by a Calcium Alginate Immobilized Mutant Strain of Aspergillus oryzae.

Author information

1
Institute of Industrial Biotechnology (IIB), GC University, Lahore, Pakistan. alisbiotech@yahoo.com.
2
Institute of Industrial Biotechnology (IIB), GC University, Lahore, Pakistan.

Abstract

The present research work is concerned with the biotransformation of L-tyrosine to dopamine (DA) by calcium alginate entrapped conidiospores of a mutant strain of Aspergillus oryzae. Different strains of A. oryzae were isolated from soil. Out of 13 isolated strains, isolate-2 (I-2) was found to be a better DA producer. The wild-type I-2 was chemically improved by treating it with different concentrations of ethyl methyl sulfonate (EMS). Among seven mutant variants, EMS-6 exhibiting maximal DA activity of 43 μg/ml was selected. The strain was further exposed with L-cysteine HCl to make it resistant against diversion and environmental stress. The conidiospores of selected mutant variant A. oryzae EMS-6 strain were entrapped in calcium alginate beads. Different parameters for immobilization were investigated. The activity was further improved from 44 to 62 μg/ml under optimized conditions (1.5 % sodium alginate, 2 ml inoculum, and 2 mm bead size). The best resistant mutant variable exhibited over threefold increase in DA activity (62 μg/ml) than did wild-type I-2 (21 μg/ml) in the reaction mixture. From the results presented in the study, it was observed that high titers of DA activity in vitro could effectively be achieved by the EMS-induced mutagenesis of filamentous fungus culture used.

KEYWORDS:

A. oryzae; Biotransformation; Calcium alginate; Dopamine; EMS

PMID:
27068833
DOI:
10.1007/s12010-016-2075-y
[Indexed for MEDLINE]

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