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Sci Rep. 2016 Apr 12;6:24084. doi: 10.1038/srep24084.

ClusterViSu, a method for clustering of protein complexes by Voronoi tessellation in super-resolution microscopy.

Andronov L1,2,3,4, Orlov I1,2,3,4, Lutz Y1,2,3,4, Vonesch JL1,2,3,4, Klaholz BP1,2,3,4.

Author information

1
Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, Illkirch, France.
2
Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France.
3
Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France.
4
Université de Strasbourg, Strasbourg, France.

Abstract

Super-resolution microscopy (PALM, STORM etc.) provides a plethora of fluorescent signals in dense cellular environments which can be difficult to interpret. Here we describe ClusterViSu, a method for image reconstruction, visualization and quantification of labelled protein clusters, based on Voronoi tessellation of the individual fluorescence events. The general applicability of this clustering approach for the segmentation of super-resolution microscopy data, including for co-localization, is illustrated on a series of important biological objects such as chromatin complexes, RNA polymerase, nuclear pore complexes and microtubules.

PMID:
27068792
PMCID:
PMC4828638
DOI:
10.1038/srep24084
[Indexed for MEDLINE]
Free PMC Article

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