Format

Send to

Choose Destination
See comment in PubMed Commons below
Neuron. 2016 Apr 20;90(2):278-91. doi: 10.1016/j.neuron.2016.03.011. Epub 2016 Apr 7.

Glycolytic Enzymes Localize to Synapses under Energy Stress to Support Synaptic Function.

Author information

1
Program in Cellular Neuroscience, Neurodegeneration, and Repair, Department of Cell Biology and Department of Neuroscience, Yale University School of Medicine, P.O. Box 9812, New Haven, CT 06536-0812, USA.
2
Department of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, UT 84112-0840, USA.
3
Laboratorio de Microbiología, Facultad de Ciencias Biológicas, Universidad Ricardo Palma, P.O. Box 1801, Lima 33, Perú
4
Program in Cellular Neuroscience, Neurodegeneration, and Repair, Department of Cell Biology and Department of Neuroscience, Yale University School of Medicine, P.O. Box 9812, New Haven, CT 06536-0812, USA; Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico, 201 Boulevard del Valle, San Juan 00901, Puerto Rico. Electronic address: daniel.colon-ramos@yale.edu.

Abstract

Changes in neuronal activity create local and transient changes in energy demands at synapses. Here we discover a metabolic compartment that forms in vivo near synapses to meet local energy demands and support synaptic function in Caenorhabditis elegans neurons. Under conditions of energy stress, glycolytic enzymes redistribute from a diffuse localization in the cytoplasm to a punctate localization adjacent to synapses. Glycolytic enzymes colocalize, suggesting the ad hoc formation of a glycolysis compartment, or a "glycolytic metabolon," that can maintain local levels of ATP. Local formation of the glycolytic metabolon is dependent on presynaptic scaffolding proteins, and disruption of the glycolytic metabolon blocks the synaptic vesicle cycle, impairs synaptic recovery, and affects locomotion. Our studies indicate that under energy stress conditions, energy demands in C. elegans synapses are met locally through the assembly of a glycolytic metabolon to sustain synaptic function and behavior. VIDEO ABSTRACT.

PMID:
27068791
PMCID:
PMC4840048
[Available on 2017-04-20]
DOI:
10.1016/j.neuron.2016.03.011
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center