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Antimicrob Agents Chemother. 2016 Jun 20;60(7):4346-50. doi: 10.1128/AAC.03072-15. Print 2016 Jul.

Initial Assessment of the Molecular Epidemiology of blaNDM-1 in Colombia.

Author information

1
Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
2
J. Craig Venter Institute, La Jolla, California, USA.
3
International Center for Medical Research and Training, CIDEIM, Cali, Colombia.
4
Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
5
J. Craig Venter Institute, La Jolla, California, USA madams@jcvi.org robert.bonomo@va.gov.
6
Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio, USA Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA madams@jcvi.org robert.bonomo@va.gov.

Abstract

We report complete genome sequences of four blaNDM-1-harboring Gram-negative multidrug-resistant (MDR) isolates from Colombia. The blaNDM-1 genes were located on 193-kb Inc FIA, 178-kb Inc A/C2, and 47-kb (unknown Inc type) plasmids. Multilocus sequence typing (MLST) revealed that these isolates belong to sequence type 10 (ST10) (Escherichia coli), ST392 (Klebsiella pneumoniae), and ST322 and ST464 (Acinetobacter baumannii and Acinetobacter nosocomialis, respectively). Our analysis identified that the Inc A/C2 plasmid in E. coli contained a novel complex transposon (Tn125 and Tn5393 with three copies of blaNDM-1) and a recombination "hot spot" for the acquisition of new resistance determinants.

PMID:
27067339
PMCID:
PMC4914651
DOI:
10.1128/AAC.03072-15
[Indexed for MEDLINE]
Free PMC Article

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