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Anal Chem. 2016 May 3;88(9):4803-7. doi: 10.1021/acs.analchem.6b00278. Epub 2016 Apr 21.

Palm-Sized Device for Point-of-Care Ebola Detection.

Author information

1
KIST Europe , Campus E7.1, 66123 Saarbrücken, Germany.
2
Northwestern Polytechnical University (NPU) , School of Mechanical Engineering, Department of Microsystem Engineering, 127 West Youyi Road, Xi'an, Shaanxi 710072, P. R. China.
3
Brno University of Technology, Central European Institute of Technology (CEITEC) and Faculty of Electrical Engineering and Communication (FEEC) , Antonínská 548/1, CZ-601 90 Brno, Czech Republic.

Abstract

We show the utilization of a recently developed cellphone-sized real-time polymerase chain reaction (PCR) device to detect Ebola virus RNA using single-step reverse transcription PCR (RT-PCR). The device was shown to concurrently perform four PCRs, each with a sample volume of 100 nL: one positive control with both Ebola and GAPDH RNA and one negative control. The last two positions were used to measure the GAPDH and the Ebola content of a sample. A comparison of threshold cycles (CT) from the two samples provided relative quantification. The entire process, which consisted of reverse transcription, PCR amplification, and melting curve analysis (MCA), was conducted in less than 37 min. The next step will be integration with a sample preparation unit to form an integrated sample-to-answer system for point-of-care infectious disease diagnostics.

PMID:
27064314
DOI:
10.1021/acs.analchem.6b00278
[Indexed for MEDLINE]

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